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pbabe puro erbb2  (Addgene inc)


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    Addgene inc pbabe puro erbb2
    Pbabe Puro Erbb2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+erbb2/pBABEpuro-ERBB2+(Plasmid+%2340978)/us12410260-161-6-7
    Average 93 stars, based on 37 article reviews
    pbabe puro erbb2 - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Highly modular biepitopic and bispecific CAR-T cells for cancer immunotherapy
    Article Snippet: .. EGFR WT (Addgene plasmid #110110) and pBABE-puro-ErbB2 (Addgene plasmid #40978) were gifts from Matthew Meyerson. ..

    Article Title: Cancer Immunotherapy with T Cells Carrying Bispecific Receptors That Mimic Antibodies
    Article Snippet: .. EGFR WT (Addgene plasmid #110110) and pBABE-puro-ErbB2 (Addgene plasmid #40978) were gifts from Matthew Meyerson. ..

    Article Title: BRAF V600E -Driven Lung Tumorigenesis Requires Ligand-Mediated Activation of ERBB Receptor Signaling
    Article Snippet: .. The following plasmids with either obtained or cloned/modified in the lab as described: pBabe-Puro-EGFR WT was a gift from Matthew Meyerson (Addgene plasmid # 11011 ; http://n2t.net/addgene:11011 ; RRID:Addgene_11011); pBABE-puro-ERBB2 was a gift from Matthew Meyerson (Addgene plasmid # 40978 ; http://n2t.net/addgene:40978 ; RRID:Addgene_40978); pMCL-HA-MAPKK1-WT was a gift from Natalie Ahn (Addgene plasmid # 40808 ; http://n2t.net/addgene:40808 ; RRID:Addgene_40808); pMCL-HA-MAPKK1-11A55B [S218E/S222D] was a gift from Natalie Ahn (Addgene plasmid # 40809; http://n2t.net/addgene:40809 ; RRID:Addgene_40809; pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454); psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260); lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961 ; http://n2t.net/addgene:52961 ; RRID:Addgene_52961). .. The following plasmids with either obtained or cloned/modified in the lab as described: pBabe-Puro-EGFR WT was a gift from Matthew Meyerson (Addgene plasmid # 11011 ; http://n2t.net/addgene:11011 ; RRID:Addgene_11011); pBABE-puro-ERBB2 was a gift from Matthew Meyerson (Addgene plasmid # 40978 ; http://n2t.net/addgene:40978 ; RRID:Addgene_40978); pMCL-HA-MAPKK1-WT was a gift from Natalie Ahn (Addgene plasmid # 40808 ; http://n2t.net/addgene:40808 ; RRID:Addgene_40808); pMCL-HA-MAPKK1-11A55B [S218E/S222D] was a gift from Natalie Ahn (Addgene plasmid # 40809; http://n2t.net/addgene:40809 ; RRID:Addgene_40809; pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454); psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260); lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961 ; http://n2t.net/addgene:52961 ; RRID:Addgene_52961).

    Article Title: Cancer immunotherapy with T cells carrying bispecific receptors that mimic antibodies
    Article Snippet: .. EGFR WT (Addgene plasmid #110110) and pBABE-puro-ErbB2 (Addgene plasmid #40978) were gifts from Matthew Meyerson. ..

    other:

    Article Title: Dihydroceramide desaturase 1 ( <scp>DES1</scp> ) promotes anchorage‐independent survival downstream of <scp>HER2</scp> ‐driven glucose uptake and metabolism
    Article Snippet: #17293), and pLenti CMV Blast DEST (Addgene #17451) were gifts from Eric Campeau

    Clone Assay:

    Article Title: BRAF V600E -Driven Lung Tumorigenesis Requires Ligand-Mediated Activation of ERBB Receptor Signaling
    Article Snippet: .. The following plasmids with either obtained or cloned/modified in the lab as described: pBabe-Puro-EGFR WT was a gift from Matthew Meyerson (Addgene plasmid # 11011 ; http://n2t.net/addgene:11011 ; RRID:Addgene_11011); pBABE-puro-ERBB2 was a gift from Matthew Meyerson (Addgene plasmid # 40978 ; http://n2t.net/addgene:40978 ; RRID:Addgene_40978); pMCL-HA-MAPKK1-WT was a gift from Natalie Ahn (Addgene plasmid # 40808 ; http://n2t.net/addgene:40808 ; RRID:Addgene_40808); pMCL-HA-MAPKK1-11A55B [S218E/S222D] was a gift from Natalie Ahn (Addgene plasmid # 40809; http://n2t.net/addgene:40809 ; RRID:Addgene_40809; pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454); psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260); lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961 ; http://n2t.net/addgene:52961 ; RRID:Addgene_52961). .. The following plasmids with either obtained or cloned/modified in the lab as described: pBabe-Puro-EGFR WT was a gift from Matthew Meyerson (Addgene plasmid # 11011 ; http://n2t.net/addgene:11011 ; RRID:Addgene_11011); pBABE-puro-ERBB2 was a gift from Matthew Meyerson (Addgene plasmid # 40978 ; http://n2t.net/addgene:40978 ; RRID:Addgene_40978); pMCL-HA-MAPKK1-WT was a gift from Natalie Ahn (Addgene plasmid # 40808 ; http://n2t.net/addgene:40808 ; RRID:Addgene_40808); pMCL-HA-MAPKK1-11A55B [S218E/S222D] was a gift from Natalie Ahn (Addgene plasmid # 40809; http://n2t.net/addgene:40809 ; RRID:Addgene_40809; pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454); psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260); lentiCRISPR v2 was a gift from Feng Zhang (Addgene plasmid # 52961 ; http://n2t.net/addgene:52961 ; RRID:Addgene_52961).



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    (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.

    Journal: bioRxiv

    Article Title: Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis

    doi: 10.1101/2024.11.19.624272

    Figure Lengend Snippet: (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.

    Article Snippet: pBABE-Puro retroviral vector (EV) and pBABE-Puro vectors carrying ERBB2 wild-type and mutants were co-transfected independently with pUMVC (Addgene #8449) and VSV-G (Addgene #8454) retroviral packaging plasmids into HEK-293T cells using PEI-transfection.

    Techniques: Transduction, Cell Culture, Co-Culture Assay, Isolation, Staining, Expressing, Mutagenesis